Mash & Lauter Science
Enzyme activity, mash temperature science, sparging strategies, and efficiency drivers for all-grain brewing
The mash is the heart of all-grain brewing. Hot water activates naturally occurring grain enzymes that break down starch into fermentable sugars, producing the wort that yeast will later ferment. The lautering step separates that wort from the spent grain. Understanding both processes lets you hit your target gravity, adjust fermentability, and maximize efficiency—brew after brew.
Enzyme Activity & the Saccharification Window
Malt contains dozens of enzymes, but two dominate wort composition during the saccharification rest:
Beta-Amylase
Beta-amylase cleaves maltose (a two-glucose sugar) from the non-reducing end of starch chains, producing highly fermentable wort.
Optimal temperature
60–65 °C (140–149 °F)
Active range
55–70 °C (131–158 °F)
Denaturation
Rapidly above 70 °C (158 °F)
Optimal pH
5.0–5.3
A mash held in the lower saccharification range favors beta-amylase, yielding more maltose, higher attenuation, and a drier, lighter-bodied beer.
Alpha-Amylase
Alpha-amylase cuts starch chains randomly, producing dextrins and longer-chain sugars (largely unfermentable by brewer's yeast) that add body and mouthfeel.
Optimal temperature
67–72 °C (153–162 °F)
Active range
60–76 °C (140–169 °F)
Denaturation
Above 76 °C (169 °F)
Optimal pH
5.3–5.7
A mash in the upper saccharification range favors alpha-amylase, producing fuller-bodied beer with lower apparent attenuation.
Practical Single-Infusion Targets
Dry, highly attenuated beer
63–65 °C (145–149 °F)
Balanced body and fermentability
66–68 °C (151–154 °F)
Full-bodied, lower attenuation
69–72 °C (156–162 °F)
A single-infusion saccharification rest of 60–90 minutes is sufficient for well-modified modern malts at any temperature in this range. Test for complete conversion with an iodine test: wort that stays golden-amber (no blue-black color change) indicates complete starch conversion.
Rule of thumb: For most modern base malts, conversion is essentially complete within 30–45 minutes at 67 °C (153 °F). Extending to 60 minutes provides a comfortable margin.
Mash pH
Mash pH strongly affects enzyme activity, wort flavor, and clarification. For practical homebrewing, target about pH 5.2–5.6 at room temperature (20 °C / 68 °F), which is often about 5.1–5.4 at mash temperature.
Below ~5.2 (room-temp reading): Enzyme activity and fermentability can drop.
~5.2–5.6 (room-temp reading): Strong overall conversion and balanced wort quality.
Above ~5.8 (room-temp reading): Increased risk of tannin/astringency pickup during lautering and sparging.
Measure mash pH on a cooled sample for consistency and meter longevity. Automatic temperature compensation (ATC) corrects electrode response, not the mash chemistry itself.
Water chemistry (alkalinity, calcium content) and grain bill (darker, more acidic malts vs. pale base malts) interact to set pH. Use Brewfather's Water Calculator to model adjustments before brew day.
Brewfather Tip 💧 Open the Water Calculator in your recipe (Recipe → Water Calculator). Enter your source water profile, grain bill, and target style. Brewfather will calculate the estimated mash pH and suggest salt additions (gypsum, calcium chloride, lactic acid, etc.) to reach your target. Save adjustments as a Water Profile for reuse.
Step Mashing
Step mashing—holding the mash at two or more progressively higher temperatures—is used for undermodified malts, high-adjunct recipes, or specific flavor goals.
Common Rest Steps
Acid rest
35–45 °C (95–113 °F)
Phytase activation; lowers mash pH (less relevant with well-modified malt)
Protein rest
45–55 °C (113–131 °F)
Protease breaks down large proteins; improves head retention, reduces haze
Beta-glucan rest
35–45 °C (95–113 °F)
Breaks down beta-glucans in oats, wheat, rye; improves lauterability
Saccharification rest
63–72 °C (145–162 °F)
Starch conversion (see above)
Mash-out
76–78 °C (169–172 °F)
Denatures enzymes; fixes fermentability; thins wort for easier lautering
Note: Most modern malts are highly modified. A protein rest is generally unnecessary for all-malt grists and can reduce head-positive proteins excessively. It remains valuable for large proportions of unmalted wheat, rye, oats, or raw grains.
Brewfather Tip 🔧 Go to Profiles → Mash to create or import a step-mash profile. Add each step (temperature + time), then assign the profile to your recipe. Brewfather will display the steps in sequence on the Brewing tab so you can track and log each step in real time during brew day.
Lautering: Converting the Mash into Wort
Lautering (German: läutern, to clarify) is the separation of sweet wort from the grain bed. It comprises three phases:
Vorlauf (recirculation): Gently recirculate the first turbid runnings back through the grain bed until the wort runs clear. This sets the grain bed as a natural filter. Typical volume: 1–4 L (1–4 qt).
First runnings: Drain the primary wort from the mash.
Sparge: Rinse the grain bed with hot water to recover residual sugars.
Sparge Methods
Fly sparge
Continuously spray hot water over the grain bed while draining at the same rate
Highest extract efficiency (typically 80–85%)
Requires equipment; longer process; tannin risk if pH rises
Batch sparge
Drain fully, add calculated sparge water, stir, wait 10–15 min, drain again
Simple; no special equipment
Slightly lower efficiency than fly sparge (typically 70–78%)
No-sparge
Mash with the full volume; drain once
Fastest; minimal risk of tannin extraction
Lowest efficiency; requires larger grain bill
Tannin Extraction Risks
Polyphenol (tannin) extraction from grain husks causes astringency. Risk rises most when high pH and high temperature overlap:
Control sparge temperature: Keep sparge liquor around 76–78 °C (168–172 °F).
Watch runoff gravity and pH: As sugars are depleted, runoff pH tends to rise. A common stopping point is around 1.008–1.010 SG runoff gravity or ~pH 5.8–6.0 (room-temp reading).
Either factor alone is usually manageable; the harshest extraction tends to happen when both drift too high late in runoff.
Mash Efficiency
Mash efficiency is the percentage of potential extract recovered into the kettle before the boil. Brewhouse efficiency is lower because it also includes kettle, transfer, and packaging losses.
Typical homebrewing ranges:
Mash efficiency: ~70–85%
Brewhouse efficiency: ~60–80%
Key efficiency drivers:
Crush quality
Ensure grain is well-crushed (husk intact, endosperm exposed); measure gap (typically 0.8–1.0 mm)
Mash pH
Stay around 5.2–5.6 (room-temp reading)
Water-to-grain ratio
2.5–4.0 L/kg (1.2–2.0 qt/lb); thinner mashes improve enzyme mobility
Mash time
60–90 min at temperature
Sparge thoroughness
More sparge water rinses more sugar
Grain bed depth
20–40 cm (8–16 in) typical for good filtration
Brewfather Tip 📊 Set your Brewhouse Efficiency in your Equipment Profile (Profiles → Equipment). Enter your actual measured efficiency from past brews. Brewfather uses this value to calculate how much grain you need to hit your target OG. After each brew, compare your measured pre-boil gravity to the Brewfather estimate and update your efficiency if needed. Over time this calibration makes your pre-brew predictions very accurate.
Mash-Out
Raising the mash to 76–78 °C (169–172 °F) for 10 minutes before lautering:
Denatures remaining enzymes, locking in the fermentability profile you've established.
Thins the wort (lower viscosity), improving flow rate through the grain bed.
Not mandatory for batch sparging or no-sparge brewing, where lautering is fast and enzyme activity has little time to continue.
Brewfather Tip 🛠️ Add a mash-out step to your Mash Profile at 76 °C for 10 min. Brewfather will include it in the step sequence and log the timestamp on your batch record.
Troubleshooting Common Mash Problems
Low pre-boil gravity (missed OG)
Poor crush, low efficiency
Check crush; calibrate efficiency in Equipment Profile
Stuck runoff / slow lautering
Fine crush, beta-glucans, collapsed grain bed
Add rice hulls; recirculate slowly; raise mash temperature
Astringent/harsh flavor
Tannin extraction (high runoff pH and/or high sparge temp)
Keep sparge at 76–78 °C; stop runoff around pH 5.8–6.0 or SG 1.008–1.010
Cloudy wort / poor clarity
Insufficient vorlauf; high mash pH
Vorlauf 2–4 L; check mash pH; use Irish moss/whirlfloc in boil
Poor fermentability (high FG)
Mash temp too high or too short
Lower mash temp; verify thermometer calibration
Sources
Palmer, J. (2017). How to Brew (4th ed.). Brewers Publications. Chapters 14–16.
Briggs, D.E., Boulton, C.A., Brookes, P.A., & Stevens, R. (2004). Brewing: Science and Practice. Woodhead Publishing.
Kunze, W. (2010). Technology Brewing and Malting (4th International ed.). VLB Berlin.
Noonan, G.J. (1996). New Brewing Lager Beer. Brewers Publications.
American Homebrewers Association. "Enzymes in Beer: What's Happening in the Mash." https://homebrewersassociation.org/how-to-brew/enzymes-in-beer-whats-happening-in-the-mash/
Lewis, A. (2017). "Setting the Record Straight on Mash pH." Brew Your Own. https://byo.com/mr-wizard/setting-record-straight-mash-ph/
Braukaiser.com. "The Theory of Mashing." https://www.braukaiser.com/wiki/index.php/The_Theory_of_Mashing
Last updated
Was this helpful?